Surgery and OncologySurgery and OncologyХирургия и онкология2949-5857Publishing House ABV Press6910.17650/2220-3478-2013-0-2-13-16IN FOCUSВ ЦЕНТРЕ ВНИМАНИЯСOLORECTAL CANCER AND INCULIN-LIKE GROWTH FACTORSКОЛОРЕКТАЛЬНЫЙ РАК И ИНСУЛИНОПОДОБНЫЕ ФАКТОРЫ РОСТАNikolayevA. A.НиколаевА. А.
Russian Federation
esgershtein@gmail.comGersteinE. S.ГерштейнЕ. С.
Russian Federation
esgershtein@gmail.comKorotkovaE. A.КоротковаЕ. А.
Russian Federation
esgershtein@gmail.comDelektorskayaV. V.ДелекторскаяВ. В.
Russian Federation
esgershtein@gmail.comDvorovaE. K.ДвороваЕ. К.
Russian Federation
esgershtein@gmail.comN.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowФГБУ «РОНЦ им. Н.Н. Блохина» РАМН, Москва270620132213162802201528022015Copyright ©; 2013, ABV-pressCopyright ©; 2013, АБВ-пресс2013ABV-pressАБВ-прессhttps://onco-surgery.info/jour/about/editorialPolicieshttps://onco-surgery.info/jour/article/view/69

Insulin-like growth factors (IGF) 1 and 2 and IGF binding proteins (IGFBP) 1 and 3 levels were measured by ELISA techniques in blood serum of 74 primary colorectal cancer (СRC) patients and 30 control practically healthy persons. Significant increase of IGF-1 level and decrease of IGFBP-3 level were demonstrated in patients’ serum as compared to control group. Sensitivity of IGF-1 as a prospective diagnostic СRС marker comprised 80 % with 75 % specificity using 140 ng/ml as cut-off level. Significant negative association was found be-tween both patients and donors’ age and serum IGF-1 levels, but in CRC patients it was much weaker than in control group. No associations were found between serum IGF 1 and 2 levels and main criteria of colorectal cancer progression.

Иммуноферментными методами определено содержание инсулиноподобных факторов роста (ИФР) 1, 2 и ИФР-связывающих белков (ИФРСБ) 1 и 3 в сыворотке крови 74 первичных больных колоректальным раком (КРР) и 30 практически здоровых доноров. Продемонстрировано достоверное повышение уровня ИФР-1 и снижение уровня ИФРСБ-3 в сыворотке больных КРР по сравнению с контролем. Чувствительность ИФР-1 как потенциального диагностического маркера КРР при пороговом уровне 140 нг/мл составляет 80 %, специфичность – 75 %. Обнаружена достоверная отрицательная корреляция между возрастом обследованных и содержанием ИФР-1, однако у больных КРР она была значительно слабее, чем в контроле. Взаимосвязи с основными показателями распространенности КРР не выявлено.

IGF-1IGF-2IGFBP-1IGFBP-3colorectal cancerИФР-1ИФР-2ИФРСБ-1ИФРСБ-3рак толстой кишки1. Костылева О.И., Герштейн Е.С., Дигаев а М.А. и др. Инсулиноподобные факторы роста, их рецепторы и связывающие белки как патогенетические факторы и потенциальные мишени терапии в онкологии. Вопросы биологической, медицинской и фармацевтической химии 2009;6:3–8.Костылева О.И., Герштейн Е.С., Дигаев а М.А. и др. Инсулиноподобные факторы роста, их рецепторы и связывающие белки как патогенетические факторы и потенциальные мишени терапии в онкологии. Вопросы биологической, медицинской и фармацевтической химии 2009;6:3–8.2. Durai R., Yang W., Gupta S. et al.The role of the insulin-like growth factor system in colorectal cancer: review of current knowledge. Int J Colorectal Dis 2005;20(3):203–20.Durai R., Yang W., Gupta S. et al.The role of the insulin-like growth factor system in colorectal cancer: review of current knowledge. Int J Colorectal Dis 2005;20(3):203–20.3. Koda M., Reszec J., Sulkowska M. et al. Expression of the insulin-like growth factor-I receptor and proapoptotic Bax and Bak proteins in human colorectal cancer. Ann NY Acad Sci 2004;1030:377–83.Koda M., Reszec J., Sulkowska M. et al. Expression of the insulin-like growth factor-I receptor and proapoptotic Bax and Bak proteins in human colorectal cancer. Ann NY Acad Sci 2004;1030:377–83.4. Fu P., Thompson J.A., Leeding K.S., Bach L.A. Insulin-like growth factors induce apoptosis as well as proliferation in LIM 1215 colon cancer cells. J Cell Biochem 2007;100(1):58–68.Fu P., Thompson J.A., Leeding K.S., Bach L.A. Insulin-like growth factors induce apoptosis as well as proliferation in LIM 1215 colon cancer cells. J Cell Biochem 2007;100(1):58–68.5. Yavari K., Taghikhani M., Maragheh M.G. et al. Knockdown of IGF-IR by RNAi inhibits SW480 colon cancer cells growth in vitro. Arch Med Res 2009;40(4):235–40.Yavari K., Taghikhani M., Maragheh M.G. et al. Knockdown of IGF-IR by RNAi inhibits SW480 colon cancer cells growth in vitro. Arch Med Res 2009;40(4):235–40.6. Davies M., Gupta S., Goldspink G., Winslet M. The insulin-like growth factor system and colorectal cancer: clinical and experimental evidence. Int J Colorectal Dis 2006;21(3):201–8.Davies M., Gupta S., Goldspink G., Winslet M. The insulin-like growth factor system and colorectal cancer: clinical and experimental evidence. Int J Colorectal Dis 2006;21(3):201–8.7. Vrieling A., Voskuil D.W., Bosma A. et al. Expression of insulin-like growth factor system components in colorectal tissue and its relation with serum IGF levels. Growth Horm IGF Res 2009;19(2):126–35.Vrieling A., Voskuil D.W., Bosma A. et al. Expression of insulin-like growth factor system components in colorectal tissue and its relation with serum IGF levels. Growth Horm IGF Res 2009;19(2):126–35.8. Sztefko K., Hodorowicz-Zaniewska D., Popiela T., Richter P. IGF-I, IGF-II, IGFBP2, IGFBP3 and acid-labile subunit (ALS) in colorectal cancer patients before surgery and during one year follow up in relation to age. Adv Med Sci 2009;54(1):51–8.Sztefko K., Hodorowicz-Zaniewska D., Popiela T., Richter P. IGF-I, IGF-II, IGFBP2, IGFBP3 and acid-labile subunit (ALS) in colorectal cancer patients before surgery and during one year follow up in relation to age. Adv Med Sci 2009;54(1):51–8.9. Bustin S.A., Dorudi S., Phillips S.M. et al. Local expression of insulin-like growth factor-I affects angiogenesis in colorectal cancer. Tumour Biol 2002;23(3):130–8.Bustin S.A., Dorudi S., Phillips S.M. et al. Local expression of insulin-like growth factor-I affects angiogenesis in colorectal cancer. Tumour Biol 2002;23(3):130–8.10. Giovannucci E. Insulin, insulin-like growth factors and colon cancer: a review of the evidence. J Nutr 2001;131(Suppl 11): 3109–20.Giovannucci E. Insulin, insulin-like growth factors and colon cancer: a review of the evidence. J Nutr 2001;131(Suppl 11): 3109–20.11. Jenab M., Riboli E., Cleveland R.J. et al. Serum C-peptide, IGFBP-1 and IGFBP-2 and risk of colon and rectal cancers in the European Prospective Investigation into Cancer and Nutrition. Int J Cancer 2007;121(2):368–76.Jenab M., Riboli E., Cleveland R.J. et al. Serum C-peptide, IGFBP-1 and IGFBP-2 and risk of colon and rectal cancers in the European Prospective Investigation into Cancer and Nutrition. Int J Cancer 2007;121(2):368–76.12. Park J.H. Inhibition of colon cancer cell growth by dietary components: role of the insulin-like growth factor (IGF) system. Asia Pac J Clin Nutr 2008;17(Suppl 1):257–60.Park J.H. Inhibition of colon cancer cell growth by dietary components: role of the insulin-like growth factor (IGF) system. Asia Pac J Clin Nutr 2008;17(Suppl 1):257–60.13. Nomura A.M., Stemmermann G.N., Lee J., Pollak M.N. Serum insulin-like growth factor I and subsequent risk of colorectal cancer among Japanese-American men. Am J Epidemiol 2003;158(5):424–31.Nomura A.M., Stemmermann G.N., Lee J., Pollak M.N. Serum insulin-like growth factor I and subsequent risk of colorectal cancer among Japanese-American men. Am J Epidemiol 2003;158(5):424–31.14. Rinaldi S., Cleveland R., Norat T. et al. Serum levels of IGF-I, IGFBP-3 and colorectal cancer risk: results from the EPIC cohort, plus a meta-analysis of prospective studies. Int J Cancer 2010;126(7):1702–15.Rinaldi S., Cleveland R., Norat T. et al. Serum levels of IGF-I, IGFBP-3 and colorectal cancer risk: results from the EPIC cohort, plus a meta-analysis of prospective studies. Int J Cancer 2010;126(7):1702–15.15. Bruchim I., Werner H. Targeting IGF-1 signaling pathways in gynecologic malignancies. Expert Opin Ther Targets 2013;17(3):307–20.Bruchim I., Werner H. Targeting IGF-1 signaling pathways in gynecologic malignancies. Expert Opin Ther Targets 2013;17(3):307–20.16. Mitsiades C.S., Mitsiades N.S., McMullan C.J. et al. Inhibition of the insulin-like growth factor receptor-1 tyrosine kinase activity as a therapeutic strategy for multiple myeloma, other hematologic malignancies, and solid tumors. Cancer Cell 2004;5(3):221–30.Mitsiades C.S., Mitsiades N.S., McMullan C.J. et al. Inhibition of the insulin-like growth factor receptor-1 tyrosine kinase activity as a therapeutic strategy for multiple myeloma, other hematologic malignancies, and solid tumors. Cancer Cell 2004;5(3):221–30.17. Perer E.S., Madan A.K., Shurin A. et al. Insulin-like growth factor I receptor antagonism augments response to chemoradiation therapy in colon cancer cells. J Surg Res 2000;94(1):1–5.Perer E.S., Madan A.K., Shurin A. et al. Insulin-like growth factor I receptor antagonism augments response to chemoradiation therapy in colon cancer cells. J Surg Res 2000;94(1):1–5.18. Maloney E.K., McLaughlin J.L., Dagdigian N.E. et al. An anti-insulin-like growth factor I receptor antibody that is a potent inhibitor of cancer cell proliferation. Cancer Res 2003;63(16):5073–83.Maloney E.K., McLaughlin J.L., Dagdigian N.E. et al. An anti-insulin-like growth factor I receptor antibody that is a potent inhibitor of cancer cell proliferation. Cancer Res 2003;63(16):5073–83.19. Vanamala J., Reddivari L., Radhakrishnan S., Tarver C. Resveratrol suppresses IGF-1 induced human colon cancer cell proliferation and elevates apoptosis via suppression of IGF-1R/Wnt and activation of p53 signaling pathways. BMC Cancer 2010;10:238.Vanamala J., Reddivari L., Radhakrishnan S., Tarver C. Resveratrol suppresses IGF-1 induced human colon cancer cell proliferation and elevates apoptosis via suppression of IGF-1R/Wnt and activation of p53 signaling pathways. BMC Cancer 2010;10:238.20. Leng S.L., Leeding K.S., Whitehead R.H., Bach L.A. Insulin-like growth factor (IGF)-binding protein-6 inhibits IGF-II-induced but not basal proliferation and adhesion of LIM 1215 colon cancer cells. Mol Cell Endocrinol 2001;174(1–2):121–7.Leng S.L., Leeding K.S., Whitehead R.H., Bach L.A. Insulin-like growth factor (IGF)-binding protein-6 inhibits IGF-II-induced but not basal proliferation and adhesion of LIM 1215 colon cancer cells. Mol Cell Endocrinol 2001;174(1–2):121–7.21. Alami N., Page V., Yu Q. et al. Recombinant human insulin-like growth factor-binding protein 3 inhibits tumor growth and targets the Akt pathway in lung and colon cancer models. Growth Horm IGF Res 2008;18(6):487–96.Alami N., Page V., Yu Q. et al. Recombinant human insulin-like growth factor-binding protein 3 inhibits tumor growth and targets the Akt pathway in lung and colon cancer models. Growth Horm IGF Res 2008;18(6):487–96.